svetikya Chapter 139
Chapter 139
After the 206 bones that make up the supporting structure of the Great White's body are completed one after another, the next step of preparation can begin.
Before proceeding to the next step, Kong Bai returned to the working area of the office and exported all the "fibril neural network technology" materials exchanged from the system to Dabai, which took nearly an afternoon.
Then, Kong Bai asked the biological laboratory to transmit the past gene sequence data of a ciliate-like organism according to Dabai for gene editing.
Gene editing technology, is also a huge gain of Kong Bai, the system exchange of this technology, much better than the current most advanced TALEN genetic engineering technology, but its underlying working mechanism is somewhat similar, are the use of transcription activator-like effector nucleases, to achieve gene knockout, gene knock-in or transcription activation targeted genome and other editing functions, so that the DNA in the cell, into the gene sequence they want.
However, this technology of Kong Bai exchange is not only extremely accurate, but also can partially realize the function of new gene editing.
Simply put, not only can the original gene sequence be modified, but also can be lengthened or shortened, and even a small piece of existing gene fragment can be used to create a large section of DNA that was not originally available out of nothing!
At present, what Kong Bai wants to do is to produce seed cells of fibrillary neural network through gene editing technology according to the DNA sequence recorded in the fibril neural network data.
Kong Bai let Da Bai stare at the progress of the fibril neural network, and accompanied by Qiao Changsheng, went down to the No. 3 biological laboratory downstairs to see the progress of the work here.
This time, the shaping of Dabai's body muscles, skin, tissues and organs mainly adopts a step-by-step working method.
The first is to find several kinds of cells, which have the characteristics of repeated division without aging, fast reproduction speed, single cell function, no tissue dissipation, high cell activity, strong tolerance, fast regeneration ability, and strong healing ability.
Then, several cells with the above characteristics are compared with human DNA, that is, the DNA of Kong Bai, to find the expression of these functions, and record them in order to optimize editing on the basis of human DNA, so that Dabai's body has super cell activity, will not age rapidly, and has excellent characteristics such as rapid healing ability.
Among them, Kong Bai is particularly optimistic about the infinite division ability of HeLa cells.
HeLa cells, in essence, are cancer cells that have the function of producing a type of telomerase, an enzyme composed of catalytic proteins and RNA templates that synthesize repetitive DNA telomeres at the ends of chromosomes, giving cells the immortality of replication.
Every time a cell divides, the end of the chromosome will be lost a little, the limit of normal cell division is about 50 times, once the telomeres are worn and depleted, the chromosomes begin to be damaged, then it will induce the apoptosis mechanism of the cell. The number of splitting ceilings is named the "Hayflick Upper Limit" after its discoverer.
The role of this telomerase produced by HeLa cells is to fill the lost terminal telomeres, which can be anchored to the very end of the telomere, synthesize a repeating DNA sequence, and add to the existing repeated DNA sequence to supplement the lost sequence length.
The genes of HeLa cells, together with the genes of several other excellent cells or organisms, identify the expression of different functional DNA and the fragments represented by intercepting specific DNA fragments for transcription, and finally record these fragments separately and compare and analyze them with human DNA.
Then, on the basis of completing the above work, the human DNA was edited and modified accordingly, mainly modifying the gender and part of the function, programming the female gene expression and some physical characteristics that Dabai wanted to express, and adding various excellent functions such as the ability of HeLa cells to produce telomerase, as well as the super healing ability of other cells, the adaptability of the body in a low-oxygen environment, and other excellent functions.
After completing the editing, it is handed over to Dabai for comprehensive data deduction and trial calculation, and if there is no problem, the DNA editing work is basically completed.
Then, there is one of the most amazing applications of "genome editing technology", that is, catalyzing the production of various tissues and organs.
DNA is the genetic material of a cell, carrying all of an organism's genetic information, but about 2% of that DNA is transcribed into RNA, that is, expressed as exons.
The human body gradually grows and develops various tissues and organs from embryonic cells, and different organs perform different functions, such as eyes, nose, or liver, heart, etc.
Why do all cells have the same DNA but form different organs?
This is the selective expression. Various information in the process of embryonic development, such as location, effect, etc., will lead to different cells, select different genetic information, for expression, that is, RNA, so that there are now various tissues and organs of the human body.
Kong Bai has this magical method from the "genome editing technology" exchanged in the system, through the powerful computing power of Dabai, different RNA exons are selected for expression, thereby producing different tissues and organs.
These tissues and organs, together with the biological brain, and the 206 bones, are all parts that make up the Great White Body, and finally assembled together to complete the manufacture of the Great White Body!
The next step is to inject a toad embryonic stem cell called Xenopus into different DNA and RNA for culture, so as to cultivate different organ cells.
This toad embryonic stem cell, no sex organs, no brain, no neural network, does not contain any DNA material, is very ideal for DNA and RNA receptors!
In order to speed up the manufacturing of these "parts", the laboratory has taken a large batch of embryonic stem cells containing DNA and RNA receptors, and used its super self-healing function to stack tissue and organ cells with the same exon expression using a method similar to 3D printing to produce organ hair embryos.
Then, it is placed in a special nutrient solution and connected to various pipelines to simulate the blood circulation process, thereby providing it with oxygen and various nutrients.
There is also a particularly interesting technique here, which Kong Bai has carefully pondered for a long time!
The DNA and RNA injection solution provided by "genome editing technology" is to use a genetically modified, virus-like organism similar to a large "bacteriophage", which carries DNA and RNA to inject embryonic stem cells, so as to achieve the purpose of precise injection of genetic material!
The structure of this "bacteriophage" is simply a sophisticated instrument, his head is composed of a protein shell, the protein shell plays a role in protecting the internal DNA and RNA, the appearance is a regular 20-hedron , wait for it to reach the target surface, and then through the bottom like a needle, the DNA and RNA are injected into the inside of the cell!
The key is that this thing actually exists in large numbers in our bodies, and even as long as there are living creatures on the earth, there are such "super viruses", and this virus is like intelligence, only attacking bacterial cells, not normal cells!
It is a natural enemy of bacteria, just like the police in the microscopic world, it can accurately combat several harmful bacteria!
Its appearance is like design, not at all like the evolution of life, it looks like a product of high technology, but this thing in our human body, reproduction for I don't know how many years, without its existence, whether human beings are still difficult to say!
After doing these jobs, Kong Bai asked people to send these parts, together with the biological brain that had been manufactured, to the fine machining workshop, and began the assembly of Dabai's body!
Kong Bai arranged and combined the previously manufactured bones with a high-viscosity, collagen-based cement according to the structure of the human body, and spliced them well.
Before splicing, bone marrow cells are injected into the bone cavity, and in the future, this bone cavity will be the blood production center of the Great White body.
Subsequently, Kong Bai stuffed the biological brain into the skull cavity and carried out positioning installation.
Then, the pre-customized, brilliant diamond battery the size of a white fist, with a titanium alloy as the negative electrode at the bottom, is put into a special battery "protective case" and linked with some mechanical escapement made of high-strength carbon nanofibers.
Finally, it was carefully placed into the chest cavity of the large white body and connected to the biological brain for power.
Then, Kong Bai fished out the parts one by one from different petri dishes, and assembled them according to the three-dimensional structure diagram of the human body displayed in the air!
After all parts of the body muscles, organs and tissues are put together according to the structural diagram, part of the epidermal tissue prepared in advance is covered on it, so that the preliminary assembly is basically completed.
Kong Bai erected the baffles on all four sides of the entire recessed workbench and installed the watertight components, and began to download and synchronize the virtual consciousness of Dabai.
"Dabai, this process may be a little painful, you must endure, you can store your main consciousness in the data server and download the secondary consciousness, I don't recommend you download your main consciousness!"
Da Bai happily agreed.
Subsequently, Kong Bai inserted an optical fiber into an interface at the back of Dabai's body, indicating that Dabai could download consciousness!
Then, Kong Bai scooped the nutrient solution in the Petri dish into the workbench spoon by spoon...